A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria
نویسنده
چکیده
Sulfide in cultures of sulfidogenic bacteria is us.ually determined by the methylene blue reaction [ I , 21. This method permits quantitative analysis of traces of dissolved sulfide (e.g., in groundwater) down to concentrations of micromoles per liter. Since this reaction takes about 20 min, the presence of dissolved sulfide in cultures of sulfatereducing bacteria (SRB) is proved rapidly by its colloidal precipitation as CuS in a copper sulfate reagent [3, 41. After its modification, this qualitative proof can be quantified photometrically, as described in this paper.
منابع مشابه
Molecular study of biofilm gene of sulfate reducing bacteria (SRB) isolated from patients with periodontitis and the effect of aloe vera plant extract on its expression by Real time-PCR method
Background and Aims: Due to the increasing problems and side effects of the use of chemical antibacterial agents as well as antibiotic resistance, this study aimed to evaluate the effects of aloe vera gel on biofilm gene expression of sulfate-reducing bacteria (SRB) isolated from patients with periodontal infection by Real time-PCR method. Materials and Methods: For this study, 100 individu...
متن کاملSulfurous Analysis of Bioelectricity Generation from Sulfate-reducing Bacteria (SRB) in a Microbial Fuel Cell
The current importance of energy emphasizes the use of renewable resources (such as wastewater) for electricity generation by microbial fuel cell (MFC). In the present study, the native sulfate-reducing bacterial strain (R.gh 3) was employed simultaneously for sulfurous component removal and bioelectricity generation. In order to enhance the electrical conductivity and provision of a compatible...
متن کاملThe Identification of proteins present in culture filtrare of Mycobacterium tuberculosis DT and comparison with human tuberculin proteins by electrophoretic methods
Abstract Background and objectives: Tuberculin is the proteins existed in tuberculosis culture medium which precipitated by trichloroacetic acid (TCA) or ammonium sulfate. Tuberculin is used for diagnosis of Tuberculosis. The aim of this study is to compare the human tuberculin produced by Razi Institute and Mycobacterium tuberculosis Culture Filtrate Protein. Material and Methods: Initially By...
متن کاملMethylation of mercury by bacteria exposed to dissolved, nanoparticulate, and microparticulate mercuric sulfides.
The production of the neurotoxic methylmercury in the environment is partly controlled by the bioavailability of inorganic divalent mercury (Hg(II)) to anaerobic bacteria that methylate Hg(II). In sediment porewater, Hg(II) associates with sulfides and natural organic matter to form chemical species that include organic-coated mercury sulfide nanoparticles as reaction intermediates of heterogen...
متن کاملMicrobial sulfate reduction and metal attenuation in pH 4 acid mine water
Sediments recovered from the flooded mine workings of the Penn Mine, a Cu-Zn mine abandoned since the early 1960s, were cultured for anaerobic bacteria over a range of pH (4.0 to 7.5). The molecular biology of sediments and cultures was studied to determine whether sulfate-reducing bacteria (SRB) were active in moderately acidic conditions present in the underground mine workings. Here we docum...
متن کامل